![]() ![]() Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death ( apoptosis). Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Immunofluorescence, where the antibody is tagged to a fluorophore, such as fluorescein or rhodamine.Chromogenic immunohistochemistry (CIH), wherein an antibody is conjugated to an enzyme, such as peroxidase (the combination being termed immunoperoxidase), that can catalyse a colour-producing reaction.Visualising an antibody-antigen interaction can be accomplished in a number of ways, mainly either of the following: Albert Coons conceptualized and first implemented the procedure in 1941. IHC takes its name from the roots "immuno", in reference to antibodies used in the procedure, and "histo", meaning tissue (compare to immunocytochemistry). It involves the process of selectively identifying antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Immunohistochemistry ( IHC) is the most common application of immunostaining. The pale wavy green area on top is the epidermis, the bottom fibrous area is the dermis. The skin is from a patient with Henoch–Schönlein purpura: IgA deposits are found in the walls of small superficial capillaries (yellow arrows). ![]() Immunofluorescence of human skin using an anti-IgA antibody. Common application of immunostaining Main staining patterns on chromogenic immunohistochemistry. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |